Exactly. This commenter also noted that the authors never really defined a lot of rather important criteria for their IHC analysis in terms of neuroanatomy and how they picked their sections to examine. Again, when the authors are unblinded, unconscious bias can easily affect selection of anatomic areas. That’s why we blind researchers in clinical research. Unfortunately, researchers in basic research often seem not to understand how important blinding is when it comes to measurements like this. Basically, this is an utterly useless paper, a waste of precious animals. Worse, it’s animal torture—and I mean that literally—for no good scientific reason.
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